Genetic analysis of lymphocyte activation by lipopolysaccharide Endotoxin
نویسندگان
چکیده
منابع مشابه
Suppression of C3H/HeJ cell activation by lipopolysaccharide endotoxin.
Earlier studies in our laboratory showed that the lipopolysaccharide (LPS) of Salmonella typhi, which fails to activate B lymphocytes of C3H/HeJ mice, can suppress proliferation and polyclonal antibody synthesis by these cells when they are stimulated by polyclonal activators. In order to determine what stage of the cell cycle was blocked, resting B cells from C3H/HeJ spleens were activated by ...
متن کاملIsolation of lipopolysaccharide endotoxin.
Endotoxins, generally produced by gram-negative bacteria, are responsible for harmful effects in many diseases. These toxic compounds of nonprotein nature are part of the intact bacterial cells as opposed to the protein exotoxins found in cell-free broth cultures. Reviews concerning endotoxins have been published by Burrows (l), and by Westphal et al. (2). This report deals with the isolation, ...
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Endotoxin neutralizing protein (ENP), a recombinant form of the anti-lipopolysaccharide factor that was isolated from amebocytes of the American horseshoe crab, Limulus polyphemus, detoxifies lipopolysaccharide (LPS) both in vitro and in vivo. Using the Limulus amebocyte lysate assay, LPS was detoxified by ENP at a 1 to 1 weight ratio (1:1). When isolated rat aortic rings were preincubated for ...
متن کاملInhibition of activated nonresponder C3H/HeJ lymphocytes by lipopolysaccharide endotoxin.
The B lymphocytes and macrophages of lipopolysaccharide (LPS) nonresponder C3H/HeJ mice were found to respond to certain R types of LPS endotoxin in a fashion resembling that ordinarily seen with the cells from normal responder mice. DNA synthesis, polyclonal antibody synthesis, and interleukin-1 activity were stimulated by Bordetella pertussis LPS and Salmonella minnesota R595 LPS, although to...
متن کاملModulating endotoxin activity by combinatorial bioengineering of meningococcal lipopolysaccharide
Neisseria meningitidis contains a very potent hexa-acylated LPS that is too toxic for therapeutic applications. We used systematic molecular bioengineering of meningococcal LPS through deletion of biosynthetic enzymes in combination with induction of LPS modifying enzymes to yield a variety of novel LPS mutants with changes in both lipid A acylation and phosphorylation. Mass spectrometry was us...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1976
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.13.6.1579-1584.1976